Objective? To analyze the effects of Danhong injection on cerebrovascular reserve (CVR), nerve function, serum protein kinase C (PKC), C-reactive protein (CRP) and pentameric protein 3 (PTX3) levels in patients with acute cerebral infarction (ACI). Methods A total of 84 patients with ACI admitted to Laixi People's Hospital from January 2019 to January 2021 were divided into the control group (42 cases, treated with Butylphthalide Sodium Chloride Injection on the basis of conventional treatment) and the observation group (42 cases, treated combined with Danhong injection on the basis of the treatment of control group). Patients in both groups were treated for 2 weeks. The clinical efficacy of patients in the two groups after treatment, the CVR , neurological function, hemorheology indexes and serological indexes of patients in the two groups before and after treatment were compared. Results? The total effective rate of the observation group (92.86%) after treatment was higher than that of the control group (69.05%); compared with before treatment, the CVR and middle cerebral artery (MCV) blood flow of patients in the two groups after treatment increased, and the observation group was higher than the control group; while the pulsation index (PI), national institutes of health stroke scale (NIHSS) score, fibrinogen (FIB) level, whole blood viscosity (WBV), erythrocyte sedimentation rate (ESR), hematocrit (HCT) and serum PKC, CRP, PTX3 levels of patients in the two groups after treatment decreased, the observation group was lower than the control group (all P <0.05). Conclusion The treatment of ACI patients with Danhong injection can effectively improve their blood hemorheology and serological index levels, thereby improving the neurological function and cerebrovascular function, and promoting the recovery of the disease.