Objective To investigate the effect of Moisture exposed burn ointment on serological indexes, psychological state and quality of life of patients with chronic refractory wounds. Methods A total of 80 cases patients with chronic refractory wounds admitted to Xi'an Hui'an Hospital from December 2019 to December 2020 were divided into the control group (40 cases) and the experimental group (40 cases), according to the random number table method. Patients in the both groups received iodophor diluent wound immersion bath, and patients in the control group were treated with Vaseline on this basis, and the patients in the experimental group were treated with Moisture exposed burn ointment on this basis, patients in the both groups continued to receive treatment for 6 months. The treatment effect, wound recovery, serological indexes, psychological state and quality of life scores before and after treatment of patients were compared between the two groups. Results After treatment, compared with the control group, the clinical total effective rate of patients in the experimental group increased, thewound healing time of patients in the experimental group shortened, the number of dressing changes and the wound visual analogue scale (VAS) score reduced, after treatment, the levels of serum tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), the scores of self-rating anxiety scale (SAS), self-rating depression scale (SDS) of patients in the two groups decreased compared with before treatment, and the experimental group was lower than the control group, the levels of serum vascular endothelial growth factor (VEGF) and the scores of physiological function, somatic pain, social function, physiological function, emotional function, mental health, vitality, and overall health score increased compared with before treatment, and the experimental group was higher than the control group (all P <0.05). Conclusion Moisture exposed burn ointment in the treatment of chronic difficulty healing wound patients, which can effectively relieve pain, shorten wound recovery time, reduce the release of inflammatory factors, and promote the expression of cell transforming growth factors, which is more conducive to promoting patient wound healing, it can relieve the anxiety and depression of patients, and improve the quality of life