Objective To explore the effects of Yinao Zengzhi acupuncture combined with Bobath rehabilitation training on the development and immune function of children with athetotic cerebral palsy. Methods 100 children with athetotic cerebral palsy admitted to Traditional Chinese Medicine Hospital of Yulin from January 2017 to January 2021 were selected and divided into the control group (50 cases) and the observation group (50 cases) according to the random number table method. The children in the control group were treated with Bobath therapy, and the children in the observation group were treated with Yinao Zengzhi acupuncture on the basis of the control group. Both groups were treated for 6 months. The clinical efficacy of the two groups of children after treatment, the development of fine motor function, the hand function of the affected side, the ability of daily living, the development of intelligence, the immune function before and after treatment, and the occurrence of clinical symptoms after treatment were compared between the two groups. Results After treatment, the total clinical effective rate in the observation group was higher than that in the control group; compared with before treatment, the scores of fine motor development quotient (FMQ), Carroll hand function rating scale, activity of daily living scale (ADL) and intelligence development scale in the two groups increased after treatment, and the observation group was higher than the control group; after treatment, the proportion of CD4+ CD25+ Foxp3+ regulatory T cells (Treg cells), the relative expression of forkhead transcription factor P3 (Foxp3) mRNA and cytotoxic T lymphocyte associated protein 4 (CTLA-4) mRNA in the two groups decreased, and the observation group was lower than the control group; after treatment, the total incidence of clinical symptoms in the observation group was lower than that in the control group (all P<0.05). Conclusion The application of Yinao Zengzhi acupuncture combined with Bobath rehabilitation training in children with athetotic cerebral palsy can effectively improve the level of intellectual development and immune function, promote the recovery of fine motor function and affected hand function, improve the ability of daily living, reduce the occurrence of clinical symptoms and improve the curative effect.