Objective? To investigate the effects of different concentrations of medical ozon autologous blood transfusion in the treatment of acute gouty arthritis and its effects on the pain degree and inflammatory factor levels of patients, so as to provide a basis for clinical treatment. Methods A total of 72 patients with acute gouty arthritis admitted to Dongguan South-East Central Hospital from June 2019 to June 2021 were selected and divided into two groups according to the random number table method. The patients in the control group (36 cases) were treated with medical ozon autologous blood transfusion with a concentration of 20 μg/mL, the patients in the study group (36 cases) were treated with medical ozon autologous blood transfusion with a concentration of 30 μg/mL, both groups were treated 10 times. The clinical efficacy, joint swelling score, pain visual analog scale (VAS) score, erythrocyte sedimentation rate (ESR), blood uric acid (BUA) levels, serum inflammatory factor levels before and after treatment, and adverse reactions were compared between the two groups. Results? The clinical total effective rate of the patients in the study group was significantly higher than that in the control group; compared with before treatment, the joint swelling score, VAS score, ESR, BUA, serum interleukin-8 (IL-8), interleukin-6 (IL-6), tumor necrosis factor-α ( TNF-α) and C-reactive protein (CRP) levels of the two groups significantly decreased, and the study group was significantly lower than the control group (P<0.05); there was no significant difference in the total incidence of adverse reactions between the two groups of patients (P>0.05). Conclusion Compared with the concentration of 20 μg/mL medical ozon autologous blood, 30 μg/mL medical ozon autologous blood reinfusion in the treatment of acute gouty arthritis can effectively improve the clinical efficacy, relieve the patient’s joint swelling and pain, and reduce body inflammation reaction, and there is no significant increase in adverse reactions.