Objective? To analyze the influence of Brinzolamide combined with Timolol on central retinal artery peak systolic velocity (PSV), end diastolic velocity (EDV), resistance index (RI), interleukin-6 (IL-6), pigment epithelial derived factor (PEDF), vascular endothelial growth factor (VEGF) in the treatment of postoperative patients with neovascular glaucoma, in order to provide an effective reference for clinical diagnosis and treatment. Methods A total of 86 patients with neovascular glaucoma who received panretinal photocoagulation +trabeculectomy from August 2020 to May 2021 in Yancheng Guze Chaoju Eye Hospital were selected and divided into control group (43 cases) and observation group (43 cases) by random number table method. Patients in the control group were treated with Timolol eye drops after operation, patients in the observation group were treated with Brinzolamide eye drops on the basis of Timolol eye drops, both groups were treated for 1 week. Intraocular pressure, average visual field sensitivity, average visual field defect, PSV, EDV, RI, IL-6, PEDF, VEGF levels before and after treatment, and the occurrence of eye pain, headache and dryness during treatment were compared between the two groups. Results After treatment, intraocular pressure, average visual field defect, RI, IL-6 and VEGF levels in the two groups were significantly lower than those before treatment, and the observation group was significantly lower than the control group, the average visual field sensitivity, PSV, EDV, PEDF levels were significantly higher than those before treatment, and the observation group was significantly higher than the control group (all P<0.05); during the treatment period, there was no significant difference in the total incidence of adverse reactions such as eye pain, headache and intraocular dryness between the two groups (P>0.05). Conclusion? Brinzolamide combined with Timolol can reduce intraocular pressure in postoperative patients with neovascular glaucoma, improve central retinal hemodynamics and retinal function, reduce the level of inflammatory factors in patients, and with good security.